The evaluation of anti-Inflammatory and anti-oxidant Activities of ethanolic extract of aristolochia Longa roots in Mice

Abstract

Aristolochia longa is one of the plants that have been used in Algerian traditional medicine and worldwide. An ethanolic extraction was performed from the roots. Determination of total polyphenols and flavonoids as well as flavonols showed considerable levels of these components in the extract but the DPPH test revealed a weak antiradical activity. The anti-inflammatory activity was investigated in vitro using red blood cell membrane stabilization method and in vivo using Carrageenan-induced oedemain mice. The plant exhibited a significant anti-inflammatory effect in vivo by reducing the size of the inflamed paw with 80.31 % but showed a low ability in stabilizing the red blood cells membranes.Some parameters of oxidative stress have been measured in the liver of miceand the results showed no effect against lipid peroxidatin caused by ROS but the GSH level was increased,that can be considered as a considerable anti-oxidant activity.These results are encouraging but another wide study is highly recommended for more perfect results.